Biotinylated Cynomolgus CD161 Protein (CD1-CM461B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Cynomolgus CD161, His Tag at 0.5μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln67-Leu227

Catalog: CD1-CM461B

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Product Description Recombinant Biotinylated Cynomolgus CD161 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln67-Leu227.
Product Category Recombinant Protein
Target CD161
Synonyms KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR
Gene/Protein ID XP_005570142.1
Species Cynomolgus
Exact Sequence Gln67-Leu227
Molecular Weight The protein has a predicted MW of 21.5 kDa. Due to glycosylation, the protein migrates to 37-42 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Cynomolgus CD161, His Tag at 0.5μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus CD161, His Tag at 0.5μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus CD161 is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus CD161 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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