Product Description |
Recombinant Biotinylated Cynomolgus CD161 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln67-Leu227. |
Product Background |
CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment. |
Product Category |
Recombinant Protein |
Target |
CD161 |
Synonyms |
KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
yes |
Amino Acid Range |
Gln67-Leu227 |
Molecular Weight |
The protein has a predicted MW of 21.5 kDa. Due to glycosylation, the protein migrates to 37-42 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Cynomolgus CD161, His Tag at 0.5ug/ml (100ul/Well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 21.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Cynomolgus CD161, His Tag at 0.5ug/ml (100ul/Well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 21.2ng/ml determined by ELISA. |
|
|
Biotinylated Cynomolgus CD161 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Cynomolgus CD161 is greater than 95% as determined by SEC-HPLC. |
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