Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)
Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)
Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)
Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln22-Asp117
Catalog #CDE-CM101B
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Product Description
 Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117.
Product Background
 CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Product Category
 Recombinant Protein
Target
 CD3E/CD3 epsilon
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Gln22-Asp117
Molecular Weight
 The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 13-16 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA.
Biotinylated Cynomolgus CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.

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