Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)
Catalog: CDE-CM101B
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Product Description | Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117. |
Product Background |
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. |
Product Category |
Recombinant Protein |
Target |
CD3E/CD3 epsilon |
Accession | |
Species |
Cynomolgus |
Amino Acid Range |
Gln22-Asp117 |
Molecular Weight | The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 13-16 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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