Name: Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)
Brand: KactusBio
SKU: CDE-CM101B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)

Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA.

Biotinylated Cynomolgus CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln22-Asp117

Catalog #CDE-CM101B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117.
Product Background	CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Product Category	Recombinant Protein / Antibody discovery

Protein	CD3E/CD3 epsilon
Synonyms	CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession	Q95LI5
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Gln22-Asp117
Molecular Weight	The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 13-16 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA.
	Biotinylated Cynomolgus CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.