Name: Biotinylated Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) (CD3-CM202B)
Brand: KactusBio
SKU: CD3-CM202B-100UG
Price: 600.0 USD
Availability: InStock

Biotinylated Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) (CD3-CM202B)

Immobilized Anti-CD3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3G, hFc Tag with the EC50 of 50.5ng/ml determined by ELISA (QC Test).

Biotinylated Cynomolgus CD3E&CD3G on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3G, hFc Tag with the EC50 of 50.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)

Catalog #CD3-CM202B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117(CD3E)&Gln23-Thr113(CD3G).
Product Background	T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery

Protein	CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms	CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g
Accession	Q95LI5.2(CD3E)&Q95LI7(CD3G)
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)
Molecular Weight	The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-CD3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3G, hFc Tag with the EC50 of 50.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-CD3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3G, hFc Tag with the EC50 of 50.5ng/ml determined by ELISA (QC Test).
	Biotinylated Cynomolgus CD3E&CD3G on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC.