• Express System: HEK293
  • Product Tag: C-His, N-Twin Strep
  • Purity: > 85% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Cynomolgus Claudin 3 Nanodisc, His-Twin Strep Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.
  • Amino Acid Range: Met1-Val220
Catalog #CLD-CM13NB
Price
Regular price $000
Regular price Sale price $000
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Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus Claudin 3 Nanodisc (CLD-CM13NB) Summary

Product Description

 Recombinant Biotinylated Cynomolgus Claudin 3 Nanodisc is expressed from HEK293 with His tag at the C-terminus and Twin Strep tag at the N-terminus.It contains Met1-Val220.

Product Background

 Claudin-3 is a memember of the Claudin family, which is present in many epithelia including skin, lung, kidney and intestine. It beautifully localizes on the cell-to-cell junctions of MCF-7 cells, clearly forming a continuous belt-like structure surrounding the cells. It has often been associated with the progression and metastasis of various tumors.

Biotinylated Cynomolgus Claudin 3 Nanodisc (CLD-CM13NB) Specifications/Details

Protein
 Claudin 3
Synonyms
 Claudin-3; CPE-R 2; CPE-receptor 2; hRVP1; CLDN3; C7orf1; CPETR2; RVP1;CLD3
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Met1-Val220
Molecular Weight
 The protein has a predicted MW of 39.58 kDa. The protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Cynomolgus Claudin 3 Nanodisc, His-Twin Strep Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.
Product Tag
 C-His, N-Twin Strep
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 85% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Cynomolgus Claudin 3 Nanodisc, His-Twin Strep Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus Claudin 3 Nanodisc, His-Twin Strep Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.
Biotinylated Cynomolgus Claudin 3 Nanodisc on Bis-Tris PAGE under reduced condition. The purity is greater than 85%. Biotinylated Cynomolgus Claudin 3 Nanodisc on Bis-Tris PAGE under reduced condition. The purity is greater than 85%.
Other Available Proteins:
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