Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein (GAT-CM401B)
Catalog: GAT-CM401B
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| Product Description | Recombinant Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1). |
Product Background |
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex. |
Product Category |
Recombinant Protein |
Target |
GARP&Latent TGF beta 1 Complex |
| Synonyms | LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1 |
Gene/Protein ID |
A0A2K5X2X9(GARP)&A0A2K5TJB2(Latent TGF beta 1) |
Species |
Cynomolgus |
Exact Sequence |
Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1) |
| Molecular Weight | The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.38ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.38ug/ml determined by ELISA. |
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The purity of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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