Name: Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein (GAT-CM401B)
Brand: KactusBio
SKU: GAT-CM401B-100UG
Price: 742.5 USD
Availability: InStock

Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein (GAT-CM401B)

Immobilized Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.

Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
Amino Acid Range: Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)

Catalog #GAT-CM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein (GAT-CM401B) Summary

Product Description	Recombinant Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of GARP.It contains Ala18-Leu628(GARP) and Leu30-Ser390(Latent TGF beta 1).
Product Background	GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein (GAT-CM401B) Specifications/Details

Protein	GARP&Latent TGF beta 1 Complex
Synonyms	LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1
Accession	A0A2K5X2X9(GARP)&A0A2K5TJB2(Latent TGF beta 1)
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Molecular Weight	The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
	Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex is greater than 90% as determined by SEC-HPLC.