Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein (GAT-CM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.38μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)

Catalog: GAT-CM401B

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Product Description Recombinant Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).
Product Category Recombinant Protein
Target GARP&Latent TGF beta 1 Complex
Synonyms LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1
Gene/Protein ID A0A2K5X2X9(GARP)&A0A2K5TJB2(Latent TGF beta 1)
Species Cynomolgus
Exact Sequence Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Molecular Weight The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Tris-Bis PAGE result.
Activity Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.38μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.38μg/ml determined by ELISA.
The purity of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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