Product Description |
Recombinant Biotinylated Cynomolgus IFN alpha/beta R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala25-Lys437. |
Product Background |
IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions.Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves. |
Product Category |
Recombinant Protein |
Target |
IFN alpha/beta R1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
yes |
Amino Acid Range |
Ala25-Lys437 |
Molecular Weight |
The protein has a predicted MW of 50.35 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.26 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated- Cynomolgus IFN alpha/beta R1, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 1.26 nM as determined in SPR assay (Biacore T200). |
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Biotinylated Cynomolgus IFN alpha/beta R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Cynomolgus IFN alpha/beta R1 is greater than 95% as determined by SEC-HPLC. |
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