Biotinylated Cynomolgus IFN alpha/beta R2 Protein (IFN-CM4R2B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ile27-Lys243
Catalog #IFN-CM4R2B
Regular price $525.00
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Product Description
Recombinant Biotinylated Cynomolgus IFN alpha/beta R2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile27-Lys243.
Product Background
Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well.
Product Category
Recombinant Protein
Target
IFN alpha/beta R2
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Ile27-Lys243
Molecular Weight
The protein has a predicted MW of 27.75 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Cynomolgus IFN alpha/beta R2, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).
Biotinylated Cynomolgus IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC.

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