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Biotinylated Cynomolgus IL-1 Beta/IL-1F2 Protein, Ultra Low Endotoxin (IL1-CE40BB-UL) Summary
Product Description |
Recombinant Biotinylated Cynomolgus IL-1 Beta/IL-1F2 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Ala117-Ser268. |
Product Background |
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Cynomolgus IL-1 Beta/IL-1F2 Protein, Ultra Low Endotoxin (IL1-CE40BB-UL) Specifications/Details
Protein |
IL-1 Beta/IL-1F2 |
Synonyms |
Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B ;Interleukin-1 β; IL-1 β; IL-1β; IL-1 β |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
yes |
Amino Acid Range |
Ala117-Ser268 |
Molecular Weight |
The protein has a predicted MW of 20.14 kDa. The protein migrates to 22-24 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-1R2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-1 Beta, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). |
Product Tag |
N-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human IL-1R2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-1 Beta, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). |
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Biotinylated Cynomolgus IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Cynomolgus IL-1 Beta is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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