Biotinylated Cynomolgus IL-17A/CTLA-8 Protein (ILA-CM417B)

Immobilized Biotinylated Cynomolgus IL-17A, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 5.6ng/ml determined by ELISA.

Biotinylated Cynomolgus IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus IL-17A is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Cynomolgus IL-17A, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 5.6ng/ml determined by ELISA.
Amino Acid Range: Gly24-Ala155

Catalog #ILA-CM417B

Price

$577⁵⁰

Size

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Biotinylated Cynomolgus IL-17A/CTLA-8 Protein (ILA-CM417B) Summary

Product Description

Recombinant Biotinylated Cynomolgus IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Gly24-Ala155.

Product Background

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

Biotinylated Cynomolgus IL-17A/CTLA-8 Protein (ILA-CM417B) Specifications/Details

Protein	IL-17A/CTLA-8
Synonyms	CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A;CTLA8; IL17CTLA8;Interleukin-17; Interleukin 17
Accession	XP_005552816.3
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Gly24-Ala155
Molecular Weight	The protein has a predicted MW of 18.02 kDa. Due to glycosylation, the protein migrates to 20-24 kDa, 26-30 kDa and 30-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Cynomolgus IL-17A, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 5.6ng/ml determined by ELISA.
Product Tag	N-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Cynomolgus IL-17A, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 5.6ng/ml determined by ELISA.
	Immobilized Biotinylated Cynomolgus IL-17A, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
	Biotinylated Cynomolgus IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus IL-17A is greater than 95% as determined by SEC-HPLC.