• Express System: E.coli
  • Product Tag: C-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus IL-18BP, His Tag (Cat. IL8-CM1BP) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-18, Avi Tag with the EC50 of 3.2ng/ml determined by ELISA.
  • Amino Acid Range: Tyr37-Asp193
Catalog #IL1-CE618B
Price
Regular price $57800
Regular price Sale price $57800
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus IL-18 Protein (IL1-CE618B) Summary

Product Description

 Recombinant Biotinylated Cynomolgus IL-18 Protein is expressed from E.coli with Avi tag at the C-terminus.It contains Tyr37-Asp193.

Product Background

 Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Biotinylated Cynomolgus IL-18 Protein (IL1-CE618B) Specifications/Details

Protein
 IL-18
Synonyms
 IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1;IL1 gamma; IL1g;Interleukin-18; Interleukin 18; IL-1; IL 1; IL1
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Tyr37-Asp193
Molecular Weight
 The protein has a predicted MW of 20.34 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus IL-18BP, His Tag (Cat. IL8-CM1BP) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-18, Avi Tag with the EC50 of 3.2ng/ml determined by ELISA.
Product Tag
 C-Avi
Fluorophore
 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus IL-18BP, His Tag (Cat. IL8-CM1BP) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-18, Avi Tag with the EC50 of 3.2ng/ml determined by ELISA. Immobilized Cynomolgus IL-18BP, His Tag (Cat. IL8-CM1BP) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-18, Avi Tag with the EC50 of 3.2ng/ml determined by ELISA.
Biotinylated Cynomolgus IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Biotinylated Cynomolgus IL-18, Avi Tag on Steptavidin-Biosensor can bind Cynomolgus IL-18 R1, His Tag (Cat. IL8-CM1R1) with an affinity constant of 4.11 nM as determined in BLI assay. Loaded Biotinylated Cynomolgus IL-18, Avi Tag on Steptavidin-Biosensor can bind Cynomolgus IL-18 R1, His Tag (Cat. IL8-CM1R1) with an affinity constant of 4.11 nM as determined in BLI assay.
The purity of Biotinylated Cynomolgus IL-18 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus IL-18 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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