Biotinylated Cynomolgus IL-2 R beta/CD122 Protein (Primary Amine Labeling) (CD1-CM122B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
  • Activity: The affinity constant of 0.148 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala27-Asp239
Catalog #CD1-CM122B
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Product Description
 Recombinant Biotinylated Cynomolgus IL-2 R beta/CD122 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Ala27-Asp239.
Product Background
 IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.
Product Category
 Recombinant Protein
Target
 IL-2 R beta/CD122
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Ala27-Asp239
Molecular Weight
 The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.148 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.148 uM as determined in SPR assay (Biacore T200).
Biotinylated Cynomolgus IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IL-2 R beta is greater than 90% as determined by SEC-HPLC.

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