- Express System: HEK293
- Product Tag: C-His
- Purity: >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
- Activity: The affinity constant of 0.148 uM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Ala27-Asp239
Product Description |
Recombinant Biotinylated Cynomolgus IL-2 R beta/CD122 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Ala27-Asp239. |
Product Background |
IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. |
Product Category |
Recombinant Protein |
Target |
IL-2 R beta/CD122 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
yes |
Amino Acid Range |
Ala27-Asp239 |
Molecular Weight |
The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.148 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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