Biotinylated Cynomolgus IL-2 R beta/CD122 Protein (Primary Amine Labeling) (CD1-CM122B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
  • Activity: The affinity constant of 0.148 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala27-Asp239
Catalog #CD1-CM122B
Regular price $525.00
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Size
image

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Product Description

Recombinant Biotinylated Cynomolgus IL-2 R beta/CD122 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Ala27-Asp239.

Product Background

IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-2 R beta/CD122

Synonyms

CD122; IL-15 R beta; IL-2 R beta; IL-2R subunit beta; IL2RB; P70-75; p75; RP5-1170K4.6; IL-15 R β; IL-2 R β; IL-2R subunit β

Accession

Species

Cynomolgus

Biotinylated

yes

Amino Acid Range

Ala27-Asp239

Molecular Weight

The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 0.148 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>90% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.148 uM as determined in SPR assay (Biacore T200). Biotinylated Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.148 uM as determined in SPR assay (Biacore T200).
Biotinylated Cynomolgus IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IL-2 R beta is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus IL-2 R beta is greater than 90% as determined by SEC-HPLC.

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