• Express System: HEK293
  • Product Tag: C-His, N-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17RB, His Tag (Cat. IL1-HM1RB) at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-25, His Tag with the EC50 of 11.1ng/ml determined by ELISA.
  • Amino Acid Range: Tyr33-Gly176
Catalog #IL2-CM425B-UL
Price
Regular price $72600
Regular price Sale price $72600
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Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus IL-25/IL-17E Protein, Ultra Low Endotoxin (IL2-CM425B-UL) Summary

Product Description

 Recombinant Biotinylated Cynomolgus IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-terminus and Avi tag at the N-terminus.It contains Tyr33-Gly176.

Product Background

 IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.

Biotinylated Cynomolgus IL-25/IL-17E Protein, Ultra Low Endotoxin (IL2-CM425B-UL) Specifications/Details

Protein
 IL-25/IL-17E
Synonyms
 IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25;IL25;Interleukin 25; IL 25
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Tyr33-Gly176
Molecular Weight
 The protein has a predicted MW of 19.99 kDa. Due to glycosylation, the protein migrates to 25-32 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-17RB, His Tag (Cat. IL1-HM1RB) at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-25, His Tag with the EC50 of 11.1ng/ml determined by ELISA.
Product Tag
 C-His, N-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-17RB, His Tag (Cat. IL1-HM1RB) at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-25, His Tag with the EC50 of 11.1ng/ml determined by ELISA. Immobilized Human IL-17RB, His Tag (Cat. IL1-HM1RB) at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-25, His Tag with the EC50 of 11.1ng/ml determined by ELISA.
Immobilized Biotinylated Cynomolgus IL-25, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti IL-25 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus IL-25, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti IL-25 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Biotinylated Cynomolgus IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IL-25 is greater than 95% and the molecular weight of this protein is around 40-65 kDa as determined by SEC-MALS. The purity of Biotinylated Cynomolgus IL-25 is greater than 95% and the molecular weight of this protein is around 40-65 kDa as determined by SEC-MALS.
The purity of Biotinylated Cynomolgus IL-25 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus IL-25 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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