• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
  • Amino Acid Range: Asn20-Ser104
Catalog #LYD-CM46DB
Price
Regular price $57800
Regular price Sale price $57800
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Biotinylated Cynomolgus LY6G6D Protein (LYD-CM46DB) Summary

Product Description

 Recombinant Biotinylated Cynomolgus LY6G6D Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Asn20-Ser104.

Product Background

 LY6G6D is a selectively expressed colorectal cancer antigen that can be used for targeting a therapeutic T-cell response by a T-cell engager.LY6G6D was identified as a selectively expressed CRC antigen that can be utilized to potently re-direct and activate cytotoxic T-cells to lyse LY6G6D expressing CRC using a TcE. This effect can be spread to target negative neighboring tumor cells, potentially leading to improved therapeutic efficacy.

Biotinylated Cynomolgus LY6G6D Protein (LYD-CM46DB) Specifications/Details

Protein
 LY6G6D
Synonyms
 Protein Ly6-D; C6orf23; G6D; MEGT1; NG25;Protein Ly6D
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Asn20-Ser104
Molecular Weight
 The protein has a predicted MW of 12.03 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Cynomolgus LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Cynomolgus LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
Biotinylated Cynomolgus LY6G6D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus LY6G6D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-LY6G6D Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus LY6G6D, His-Avi Tag with an affinity constant of 1.55 nM as determined in BLI assay. Loaded Anti-LY6G6D Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus LY6G6D, His-Avi Tag with an affinity constant of 1.55 nM as determined in BLI assay.
The purity of Biotinylated Cynomolgus LY6G6D is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus LY6G6D is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...