Name: Biotinylated Cynomolgus MADCAM1 Protein (MCM-CM401B)
Brand: KactusBio
SKU: MCM-CM401B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Cynomolgus MADCAM1 Protein (MCM-CM401B)

Immobilized Biotinylated Cynomolgus MADCAM1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.

Biotinylated Cynomolgus MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus MADCAM1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Cynomolgus MADCAM1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
Amino Acid Range: Gln22-Gln295

Catalog #MCM-CM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Cynomolgus MADCAM1 Protein (MCM-CM401B) Summary

Product Description	Recombinant Biotinylated Cynomolgus MADCAM1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gln22-Gln295.
Product Background	Mucosal addressin cell adhesion molecule–1 (MAdCAM-1) is an endothelial cell marker that is recognized by its ligands on lymphocytes: L-selectin and α4β7 integrin. MAdCAM-1 is highly expressed on intestinal endothelium and has, recently, been shown to play an important role in the development of acute GVHD in animal model.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Cynomolgus MADCAM1 Protein (MCM-CM401B) Specifications/Details

Protein	MADCAM1
Synonyms	MAdCAM-1; MADCAM1; MACAM1
Accession	XP_045235080.2
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Gln22-Gln295
Molecular Weight	The protein has a predicted MW of 31.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Cynomolgus MADCAM1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Cynomolgus MADCAM1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
	Biotinylated Cynomolgus MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus MADCAM1 is greater than 95% as determined by SEC-HPLC.