Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-CM110B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe19-Arg238
Catalog #PDL-CM110B
Regular price $525.00
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Product Description

Recombinant Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Arg238.

Product Background

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

PD-L1/B7-H1

Synonyms

CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1

Accession

Species

Cynomolgus/Rhesus macaque

Biotinylated

yes

Amino Acid Range

Phe19-Arg238

Molecular Weight

The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Biotinylated Cynomolgus/Rhesus macaque PD-L1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Cynomolgus/Rhesus macaque PD-L1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus/Rhesus macaque PD-L1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus/Rhesus macaque PD-L1 is greater than 95% as determined by SEC-HPLC.

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