Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-CM110B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe19-Arg238
Catalog #PDL-CM110B
Regular price $577.50
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Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-CM110B) Summary

Product Description

Recombinant Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Arg238.

Product Background

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-CM110B) Specifications/Details

Protein
PD-L1/B7-H1
Synonyms
CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Accession
Species
Cynomolgus/Rhesus macaque
Biotinylated
yes
Amino Acid Range
Phe19-Arg238
Molecular Weight
The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Biotinylated Cynomolgus/Rhesus macaque PD-L1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Cynomolgus/Rhesus macaque PD-L1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus/Rhesus macaque PD-L1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus/Rhesus macaque PD-L1 is greater than 95% as determined by SEC-HPLC.
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