Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-CM410B-UL)

SKU: PSM-CM410B-UL-100UG

Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-CM410B-UL)

SKU: PSM-CM410B-UL-100UG
Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-CM410B-UL)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus PSMA, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >350 pmol/min/µg.
  • Amino Acid Range: Lys44-Ala750
Catalog #PSM-CM410B-UL
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Size
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Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-CM410B-UL) Summary

Product Description

 Recombinant Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer) is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Lys44-Ala750.

Product Background

 Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets

Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-CM410B-UL) Specifications/Details

Protein
 PSMA/FOLH1
Synonyms
 GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Lys44-Ala750
Molecular Weight
 The protein has a predicted MW of 83.34 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Cynomolgus PSMA, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >350 pmol/min/µg.
Product Tag
 N-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 50mM MES, 150mM NaCl, 8% trehalose (pH 6.0).
Reconstitution
 Dissolve the lyophilized protein in 50mM MES (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Cynomolgus PSMA, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus PSMA, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
Biotinylated Cynomolgus PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus PSMA is greater than 95% and the molecular weight of this protein is around 180-220 kDa as determined by SEC-MALS. The purity of Biotinylated Cynomolgus PSMA is greater than 95% and the molecular weight of this protein is around 180-220 kDa as determined by SEC-MALS.
The purity of Biotinylated Cynomolgus PSMA is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus PSMA is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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