• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus/Rhesus macaque B7-H5, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
  • Amino Acid Range: Phe33-Thr192
Catalog #BH7-CM475B
Price
Regular price $578.00
Regular price Sale price $578.00
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus/Rhesus macaque B7-H5/Gi24/VISTA Protein (BH7-CM475B) Summary

Product Description

 Recombinant Biotinylated Cynomolgus/Rhesus macaque B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Phe33-Thr192.

Product Background

 B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.

Product Category

 Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Cynomolgus/Rhesus macaque B7-H5/Gi24/VISTA Protein (BH7-CM475B) Specifications/Details

Protein
 B7-H5/Gi24/VISTA
Synonyms
 B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1
Accession
Species
 Cynomolgus/Rhesus macaque
Biotinylated
 yes
Amino Acid Range
 Phe33-Thr192
Molecular Weight
 The protein has a predicted MW of 20.94 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Cynomolgus/Rhesus macaque B7-H5, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Cynomolgus/Rhesus macaque B7-H5, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus/Rhesus macaque B7-H5, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
Biotinylated Cynomolgus/Rhesus macaque B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus/Rhesus macaque B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus/Rhesus macaque B7-H5 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus/Rhesus macaque B7-H5 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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