• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Cynomolgus/Rhesus macaque TNFSF15, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DR3, hFc Tag (Cat. DR3-HM603) with the EC50 of 0.15ug/ml determined by ELISA.
  • Amino Acid Range: Leu72-Leu251
Catalog #FSF-CM415B
Price
Regular price $57800
Regular price Sale price $57800
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus/Rhesus macaque TNFSF15 Protein (FSF-CM415B) Summary

Product Description

 Recombinant Biotinylated Cynomolgus/Rhesus macaque TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Leu72-Leu251.

Product Background

 TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Biotinylated Cynomolgus/Rhesus macaque TNFSF15 Protein (FSF-CM415B) Specifications/Details

Protein
 TNFSF15
Synonyms
 TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A; TNF superfamily member 15;VEGI251
Accession
Species
 Cynomolgus/Rhesus macaque
Biotinylated
 yes
Amino Acid Range
 Leu72-Leu251
Molecular Weight
 The protein has a predicted MW of 23.33 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Cynomolgus/Rhesus macaque TNFSF15, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DR3, hFc Tag (Cat. DR3-HM603) with the EC50 of 0.15ug/ml determined by ELISA.
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Cynomolgus/Rhesus macaque TNFSF15, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DR3, hFc Tag (Cat. DR3-HM603) with the EC50 of 0.15ug/ml determined by ELISA. Immobilized Biotinylated Cynomolgus/Rhesus macaque TNFSF15, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DR3, hFc Tag (Cat. DR3-HM603) with the EC50 of 0.15ug/ml determined by ELISA.
Immobilized Biotinylated Cynomolgus/Rhesus macaque TNFSF15, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus/Rhesus macaque TNFSF15, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
Immobilized Biotinylated Cynomolgus/Rhesus macaque TNFSF15, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DcR3, hFc Tag with the EC50 of 20.6ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus/Rhesus macaque TNFSF15, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DcR3, hFc Tag with the EC50 of 20.6ng/ml determined by ELISA.
Biotinylated Cynomolgus/Rhesus macaque TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Biotinylated Cynomolgus/Rhesus macaque TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Other Available Proteins:
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