Name: Biotinylated Cynomolgus Siglec-15/CD33L3 Protein (SIG-CM415B)
Brand: KactusBio
SKU: SIG-CM415B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve of biotinylated cynomolgus Siglec-15 His-tag protein showing OD450 response to anti-human Siglec-15 hFc-tagged antibody.

Biotinylated Cynomolgus Siglec-15/CD33L3 Protein (SIG-CM415B)

Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).

Biotinylated Cynomolgus Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus Siglec-15 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Met60-Thr322

Catalog #SIG-CM415B

Size

100UG

500UG

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Biotinylated Cynomolgus Siglec-15/CD33L3 Protein (SIG-CM415B) Summary

Product Description	Recombinant Biotinylated Cynomolgus Siglec-15/CD33L3 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Met60-Thr322.
Product Background	Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Cynomolgus Siglec-15/CD33L3 Protein (SIG-CM415B) Specifications/Details

Protein	Siglec-15/CD33L3
Synonyms	CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
Accession	XP_005586866.2
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Met60-Thr322
Molecular Weight	The protein has a predicted MW of 29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM PB, 500mM NaCl, 0.1M L-arginine (pH 6.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
	Biotinylated Cynomolgus Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus Siglec-15 is greater than 95% as determined by SEC-HPLC.