Biotinylated Cynomolgus Siglec-15/CD33L3 Protein (SIG-CM415B)
Biotinylated Cynomolgus Siglec-15/CD33L3 Protein (SIG-CM415B)
Biotinylated Cynomolgus Siglec-15/CD33L3 Protein (SIG-CM415B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Met60-Thr322
Catalog #SIG-CM415B
Regular price $525.00
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Product Description
Recombinant Biotinylated Cynomolgus Siglec-15/CD33L3 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Met60-Thr322.
Product Background
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Product Category
Recombinant Protein
Target
Siglec-15/CD33L3
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Met60-Thr322
Molecular Weight
The protein has a predicted MW of 29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in 20mM PB, 500mM NaCl, 0.1M L-arginine (pH 6.0).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Biotinylated Cynomolgus Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus Siglec-15 is greater than 95% as determined by SEC-HPLC.

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