• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Cynomolgus SOST, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 37.6ng/ml determined by ELISA.
  • Amino Acid Range: Gln69-Tyr258
Catalog #SOT-CM401B
Price
Regular price $57800
Regular price Sale price $57800
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus SOST/Sclerostin Protein (SOT-CM401B) Summary

Product Description

 Recombinant Biotinylated Cynomolgus SOST/Sclerostin Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gln69-Tyr258.

Product Background

 SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Biotinylated Cynomolgus SOST/Sclerostin Protein (SOT-CM401B) Specifications/Details

Protein
 SOST/Sclerostin
Synonyms
 sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Gln69-Tyr258
Molecular Weight
 The protein has a predicted MW of 24.39 kDa. Due to glycosylation, the protein migrates to 32-38 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Cynomolgus SOST, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 37.6ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Cynomolgus SOST, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 37.6ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus SOST, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 37.6ng/ml determined by ELISA.
Immobilized Biotinylated Cynomolgus SOST, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus SOST, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA.
Biotinylated Cynomolgus SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...