Biotinylated Cynomolgus Transferrin R/CD71 Protein, Ultra Low Endotoxin (TFR-CM401B-UL)

SKU: TFR-CM401B-UL-100UG

Biotinylated Cynomolgus Transferrin R/CD71 Protein, Ultra Low Endotoxin (TFR-CM401B-UL)

SKU: TFR-CM401B-UL-100UG
Biotinylated Cynomolgus Transferrin R/CD71 Protein, Ultra Low Endotoxin (TFR-CM401B-UL)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human Transferrin, His Tag (Cat. TFN-HM101) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus Transferrin R, His Avi Tag with the EC50 of 44.4ng/ml determined by ELISA.
  • Amino Acid Range: Cys89-Phe760
Catalog #TFR-CM401B-UL
Regular price $578.00
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Biotinylated Cynomolgus Transferrin R/CD71 Protein, Ultra Low Endotoxin (TFR-CM401B-UL) Summary

Product Description

Recombinant Biotinylated Cynomolgus Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Cys89-Phe760.

Product Background

The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Product Category

Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Cynomolgus Transferrin R/CD71 Protein, Ultra Low Endotoxin (TFR-CM401B-UL) Specifications/Details

Protein
Transferrin R/CD71
Synonyms
TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Cys89-Phe760
Molecular Weight
The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Transferrin, His Tag (Cat. TFN-HM101) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus Transferrin R, His Avi Tag with the EC50 of 44.4ng/ml determined by ELISA.
Product Tag
N-His-Avi

Fluorophore

Biotin-labeled
Expression System
HEK293
Purity
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Transferrin, His Tag (Cat. TFN-HM101) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus Transferrin R, His Avi Tag with the EC50 of 44.4ng/ml determined by ELISA. Immobilized Human Transferrin, His Tag (Cat. TFN-HM101) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus Transferrin R, His Avi Tag with the EC50 of 44.4ng/ml determined by ELISA.
Biotinylated Cynomolgus Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Biotinylated Cynomolgus Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Biotinylated Cynomolgus Transferrin R is greater than 90% and the molecular weight of this protein is around 160-180 kDa as determined by SEC-MALS. The purity of Biotinylated Cynomolgus Transferrin R is greater than 90% and the molecular weight of this protein is around 160-180 kDa as determined by SEC-MALS.
The purity of Biotinylated Cynomolgus Transferrin R is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus Transferrin R is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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