Biotinylated Cynomolgus TSLP Protein (TSP-CM402B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus TSLP, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
  • Amino Acid Range: Tyr29-Gln159
Catalog #TSP-CM402B
Regular price $578.00
/
Size

Order by 4pm ET for delivery on:

Biotinylated Cynomolgus TSLP Protein (TSP-CM402B) Summary

Product Description

Recombinant Biotinylated Cynomolgus TSLP Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Tyr29-Gln159.

Product Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Cynomolgus TSLP Protein (TSP-CM402B) Specifications/Details

Protein
TSLP
Synonyms
thymic stromal lymphopoietin; TSLP
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Tyr29-Gln159
Molecular Weight
The protein has a predicted MW of 18.1 kDa. Due to the furin cleavage site, the protein migrates to 8-12 kDa, 18-22 kDa and 25-30 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Cynomolgus TSLP, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
Product Tag
C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Cynomolgus TSLP, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus TSLP, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
Immobilized Biotinylated Cynomolgus TSLP, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus TSLP, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Biotinylated Cynomolgus TSLP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus TSLP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus TSLP is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus TSLP is greater than 95% as determined by SEC-HPLC.
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Recommended Products

Recently viewed