Name: Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein (MHC-HM454B)
Brand: KactusBio
SKU: MHC-HM454B-100UG
Price: 619.3 USD
Availability: InStock

ELISA binding curve of biotinylated HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) His-tagged complex showing OD450 response versus protein concentration.

Immobilized HLA-A*11:01&B2M&KRAS G12D TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.1ug/ml determined by ELISA.

Biotinylated HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized HLA-A*11:01&B2M&KRAS G12D TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.1ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*11:01), Ile21-Met119 (B2M) and VVVGADGVGK peptide

Catalog #MHC-HM454B

Size

100UG

500UG

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Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein (MHC-HM454B) Summary

Product Description	Recombinant Biotinylated Human HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein is expressed from E.coli with with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A11:01), Ile21-Met119 (B2M) and VVVGADGVGK peptide.
Product Background	Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Specifications

Protein	HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK)
Synonyms	MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
MHC Form	Monomer
Allele	HLA-A*11:01
Accession	AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*11:01), Ile21-Met119 (B2M) and VVVGADGVGK peptide
Molecular Weight	The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity	Immobilized HLA-A11:01&B2M&KRAS G12D TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.1ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized HLA-A11:01&B2M&KRAS G12D TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.1ug/ml determined by ELISA.
	Biotinylated HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer is greater than 95% as determined by SEC-HPLC.