Name: Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B)
Brand: KactusBio
SKU: 2B4-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B)

Immobilized Biotinylated Human 2B4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).

Biotinylated Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human CD48, hFc Tag on ProA-Biosensor can bind Biotinylated Human 2B4, His-Avi Tag with an affinity constant of 24.90 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human 2B4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Amino Acid Range: Cys22-Arg221

Catalog #2B4-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B) Summary

Product Description	Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys22-Arg221.
Product Background	2B4 (CD244) is expressed by memory-phenotype CD8(+) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B) Specifications/Details

Protein	2B4/CD244/SLAMF4
Synonyms	2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
Accession	Q9BZW8-2
Species	Human
Biotinylated	yes
Amino Acid Range	Cys22-Arg221
Molecular Weight	The protein has a predicted MW of 24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human 2B4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human 2B4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
	Biotinylated Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human CD48, hFc Tag on ProA-Biosensor can bind Biotinylated Human 2B4, His-Avi Tag with an affinity constant of 24.90 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC.