Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human 2B4, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD48, hFc Tag with the Ec50 of 7.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Cys22-Arg221

Catalog: 2B4-HM401B

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Product Description Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Cys22-Arg221.
Product Category Recombinant Protein
Target 2B4/CD244/SLAMF4
Synonyms 2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
Gene/Protein ID Q9BZW8-2
Species Human
Exact Sequence Cys22-Arg221
Molecular Weight The protein has a predicted MW of 24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human 2B4, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD48, hFc Tag with the Ec50 of 7.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human 2B4, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD48, hFc Tag with the Ec50 of 7.2ng/ml determined by ELISA.
The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human 2B4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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