Name: Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B)
Brand: KactusBio
SKU: 2B4-HM401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B)

Immobilized Biotinylated Human 2B4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA.

Biotinylated Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human 2B4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Cys22-Arg221

Catalog #2B4-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys22-Arg221.
Product Background	2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	2B4/CD244/SLAMF4
Synonyms	2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
Accession	Q9BZW8-2
Species	Human
Biotinylated	yes
Amino Acid Range	Cys22-Arg221
Molecular Weight	The protein has a predicted MW of 24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human 2B4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human 2B4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA.
	Biotinylated Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC.