Product Description |
Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys22-Arg221. |
Product Background |
2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness. |
Product Category |
Recombinant Protein |
Target |
2B4/CD244/SLAMF4 |
Synonyms |
2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Cys22-Arg221 |
Molecular Weight |
The protein has a predicted MW of 24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human 2B4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human 2B4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA. |
|
|
Biotinylated Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC. |
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