Name: Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Brand: KactusBio
SKU: BBL-HM241B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve of biotinylated human 4-1BB ligand (trimer) interacting with hFc-tagged human 4-1BB, showing OD450 response.

Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)

Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.

Biotinylated Human 4-1BB Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-monomeric hFc, N-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Arg71-Glu254

Catalog #BBL-HM241B

Size

100UG

500UG

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Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B) Summary

Product Description	Recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with Flag tag and monomeric hFc tag at the N-Terminus.It contains Arg71-Glu254 trimer design.
Product Background	The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B) Specifications/Details

Protein	4-1BB Ligand/TNFSF9
Synonyms	41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Accession	P41273
Species	Human
Biotinylated	yes
Amino Acid Range	Arg71-Glu254
Molecular Weight	The protein has a predicted MW of 84.3 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-monomeric hFc, N-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
	Biotinylated Human 4-1BB Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 90% as determined by SEC-HPLC.