Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)

  • Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg71-Glu254

Catalog: BBL-HM241B

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Product Description Recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Arg71-Glu254.

Product Background

 The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

Product Category

 Recombinant Protein

Target

 4-1BB Ligand/TNFSF9 Trimer
Synonyms 41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9

Gene/Protein ID

 P41273

Species

 Human

Exact Sequence

 Arg71-Glu254
Molecular Weight The protein has a predicted MW of 84.3 kDa same as Tris-Bis PAGE result.

Activity

 Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-monomeric hFc

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 90% as determined by SEC-HPLC.
Biotinylated Human 4-1BB Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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