Name: Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Brand: KactusBio
SKU: BBL-HM241B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)

Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.

Biotinylated Human 4-1BB Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-monomeric hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Arg71-Glu254

Catalog #BBL-HM241B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Arg71-Glu254.
Product Background	The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	4-1BB Ligand/TNFSF9 Trimer
Synonyms	41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Accession	P41273
Species	Human
Biotinylated	yes
Amino Acid Range	Arg71-Glu254
Molecular Weight	The protein has a predicted MW of 84.3 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-monomeric hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
	Biotinylated Human 4-1BB Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 90% as determined by SEC-HPLC.