Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) (BBL-HM241B)
  • Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg71-Glu254
Catalog #BBL-HM241B
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Product Description
 Recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Arg71-Glu254.
Product Background
 The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
Product Category
 Recombinant Protein
Target
 4-1BB Ligand/TNFSF9 Trimer
Synonyms
 41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Arg71-Glu254
Molecular Weight
 The protein has a predicted MW of 84.3 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-monomeric hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
Biotinylated Human 4-1BB Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 90% as determined by SEC-HPLC.

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