Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM441B)
Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM441B)
Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM441B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu24-Gln186
Catalog #BB4-HM441B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gln186.
Product Background
4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
Product Category
Recombinant Protein
Target
4-1BB/TNFRSF9
Synonyms
CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu24-Gln186
Molecular Weight
The protein has a predicted MW of 20.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.

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