Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM441B)
Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM441B)
Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM441B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu24-Gln186
Catalog #BB4-HM441B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gln186.

Product Background

4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

4-1BB/TNFRSF9

Synonyms

CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Leu24-Gln186

Molecular Weight

The protein has a predicted MW of 20.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test).
Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.

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