Product Description |
Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gln186. |
Product Background |
4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. |
Product Category |
Recombinant Protein |
Target |
4-1BB/TNFRSF9 |
Synonyms |
CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu24-Gln186 |
Molecular Weight |
The protein has a predicted MW of 20.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). |
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Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC. |
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