Name: Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541B)
Brand: KactusBio
SKU: BB4-HM541B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541B)

Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA.

Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu24-Gln186

Catalog #BB4-HM541B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Leu24-Gln186.
Product Background	4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	4-1BB/TNFRSF9
Synonyms	CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Accession	Q07011
Species	Human
Biotinylated	yes
Amino Acid Range	Leu24-Gln186
Molecular Weight	The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA.
	Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.