Name: Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541B)
Brand: KactusBio
SKU: BB4-HM541B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541B)

Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA.

Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.21 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA.
Amino Acid Range: Leu24-Gln186

Catalog #BB4-HM541B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541B) Summary

Product Description	Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu24-Gln186.
Product Background	4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541B) Specifications/Details

Protein	4-1BB/TNFRSF9
Synonyms	CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Accession	Q07011
Species	Human
Biotinylated	yes
Amino Acid Range	Leu24-Gln186
Molecular Weight	The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA.
	Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.21 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.