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Biotinylated Human 4-1BB/TNFRSF9 Protein, Ultra Low Endotoxin (BB4-HM541B-UL) Summary
Product Description |
Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu24-Gln186. |
Product Background |
4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Biotinylated Human 4-1BB/TNFRSF9 Protein, Ultra Low Endotoxin (BB4-HM541B-UL) Specifications/Details
Protein |
4-1BB/TNFRSF9 |
Synonyms |
CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu24-Gln186 |
Molecular Weight |
The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human 4-1BB, hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer), His Tag (Cat. BBL-HM141) with the EC50 of 16.8ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1)-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human 4-1BB, hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer), His Tag (Cat. BBL-HM141) with the EC50 of 16.8ng/ml determined by ELISA. |
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Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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