Product Description |
Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Gln18-Ser740. |
Product Background |
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. |
Product Category |
Recombinant Protein |
Target |
ACE2/ACEH |
Synonyms |
ACE-2; ACEH; ACE2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln18-Ser740 |
Molecular Weight |
The protein has a predicted MW of 111.2 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized SARS-COV-2 Spike S, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized SARS-COV-2 Spike S, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. |
|
|
Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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