Biotinylated Human ACE2/ACEH Protein (ACE-HM501B)

  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized SARS-COV-2 Spike S, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln18-Ser740

Catalog: ACE-HM501B

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Product Description Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.

Product Background

 ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.

Product Category

 Recombinant Protein

Target

 ACE2/ACEH
Synonyms ACE-2; ACEH; ACE2

Gene/Protein ID

 Q9BYF1-1

Species

 Human

Exact Sequence

 Gln18-Ser740
Molecular Weight The protein has a predicted MW of 111.2 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized SARS-COV-2 Spike S, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.

Product Tag

 C-hFc-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA.
Biotinylated Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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