Biotinylated Human AFP (HLA-A*02:03) Protein (MHC-HM432B)
Catalog: MHC-HM432B
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| Product Description | Recombinant Biotinylated Human AFP (HLA-A*02:03) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal..It contains Gly25-Thr305(HLA-A*02:03),Ile21-Met119(B2M) and FMNKFIYEI peptide. |
| Product Category | Recombinant Protein |
| Target | AFP (HLA-A*02:03) |
| Gene/Protein ID | AAA03604.1(HLA-A*02:03)&P61769(B2M)&FMNKFIYEI |
| Species | Human |
| Exact Sequence | Gly25-Thr305(HLA-A*02:03),Ile21-Met119(B2M)and FMNKFIYEI peptide |
| Molecular Weight | The protein has a predicted MW of 50.70 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Biotinylated Human AFP (HLA-A*02:03) is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human AFP (HLA-A*02:03) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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