Product Description |
Recombinant Biotinylated Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide. |
Product Background |
Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
Protein |
HLA-A*02:03&B2M&AFP (FMNKFIYEI) |
Synonyms |
Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC |
MHC Form |
Monomer |
Allele |
HLA-A*02:03 |
Accession |
AAA03604.1(HLA-A*02:03)&P61769(B2M)&FMNKFIYEI |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide |
Molecular Weight |
The protein has a predicted MW of 50.70 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Additional Information
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed