Biotinylated Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer Protein (MHC-HM432B)
Biotinylated Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer Protein (MHC-HM432B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide
Catalog #MHC-HM432B
Regular price $310.00
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Product Description

Recombinant Biotinylated Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide.

Product Background

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

Product Category

Recombinant Protein / Antibody discovery / MHC

Protein

HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer

Synonyms

Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC

MHC Form

Monomer

Allele

HLA-A*02:03

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide

Molecular Weight

The protein has a predicted MW of 50.70 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC.

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