Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM403B)
Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM403B)
Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM403B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ile20-Asp503
Catalog #APE-HM403B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile20-Asp503.
Product Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category
Recombinant Protein
Target
Alkaline Phosphatase (Germ type)/ALPG
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ile20-Asp503
Molecular Weight
The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA.
Biotinylated Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Alkaline Phosphatase (Germ type) is greater than 90% as determined by SEC-HPLC.

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