Product Description |
Recombinant Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile20-Asp503. |
Product Background |
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
Product Category |
Recombinant Protein |
Target |
Alkaline Phosphatase (Germ type)/ALPG |
Synonyms |
Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ile20-Asp503 |
Molecular Weight |
The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA. |
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Biotinylated Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human Alkaline Phosphatase (Germ type) is greater than 90% as determined by SEC-HPLC. |
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