Name: Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM403B)
Brand: KactusBio
SKU: APE-HM403B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM403B)

Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA.

Biotinylated Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Alkaline Phosphatase (Germ type) is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ile20-Asp503

Catalog #APE-HM403B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile20-Asp503.
Product Background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category	Recombinant Protein / Antibody discovery

Protein	Alkaline Phosphatase (Germ type)/ALPG
Synonyms	Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2
Accession	P10696
Species	Human
Biotinylated	yes
Amino Acid Range	Ile20-Asp503
Molecular Weight	The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA.
	Biotinylated Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Alkaline Phosphatase (Germ type) is greater than 90% as determined by SEC-HPLC.