Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (APE-HM102B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ile23-Asp506
Catalog #APE-HM102B
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Product Description
 Recombinant Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains Ile23-Asp506.
Product Background
 Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category
 Recombinant Protein
Target
 Alkaline Phosphatase (Placental type)/ALPP
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ile23-Asp506
Molecular Weight
 The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Biotinylated Human Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.
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