Name: Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (APE-HM102B)
Brand: KactusBio
SKU: APE-HM102B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (APE-HM102B)

Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).

Biotinylated Human Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ile23-Asp506

Catalog #APE-HM102B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains Ile23-Asp506.
Product Background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category	Recombinant Protein / Antibody discovery

Protein	Alkaline Phosphatase (Placental type)/ALPP
Synonyms	Alkaline phosphatase; PLAP-1; ALPP; PLAP
Accession	P05187
Species	Human
Biotinylated	yes
Amino Acid Range	Ile23-Asp506
Molecular Weight	The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
	Biotinylated Human Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.