Product Description |
Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Lys275-Phe496. |
Product Background |
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. |
Product Category |
Recombinant Protein |
Target |
ANGPT2/Angiopoietin-2 |
Synonyms |
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Lys275-Phe496 |
Molecular Weight |
The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human ANGPT2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 2.93 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
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Human TIE2, His Tag immobilized on CM5 Chip can bind Biotinylated Human ANGPT2, His Tag with an affinity constant of 2.93 uM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human ANGPT2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). |
|
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Biotinylated Human ANGPT2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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