Biotinylated Human ANGPT2/Angiopoietin-2 Protein (APN-HM401B)
Catalog: APN-HM401B
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| Product Description | Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Lys275-Phe496. |
Product Background |
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. |
Product Category |
Recombinant Protein |
Target |
ANGPT2/Angiopoietin-2 |
| Synonyms | AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand |
Gene/Protein ID |
O15123-1 |
Species |
Human |
Exact Sequence |
Lys275-Phe496 |
| Molecular Weight | The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-ANGPT2 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPT2, His Tag with the EC50 of 0.12ug/ml determined by ELISA. The affinity constant of 2.93 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human TIE2, His Tag immobilized on CM5 Chip can bind Biotinylated Human ANGPT2, His Tag with an affinity constant of 2.93 uM as determined in SPR assay (Biacore T200). |
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Immobilized Anti-ANGPT2 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPT2, His Tag with the EC50 of 0.12ug/ml determined by ELISA. |
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Biotinylated Human ANGPT2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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