Biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein (ANG-HM4L2B)
Catalog: ANG-HM4L2B
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| Product Description | Recombinant Biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr260-His493. |
Product Background |
Angiopoietin-like 2 (ANGPTL2) is a proinflammatory protein belonging to the angiopoietin-like family. ANGPTL2 is secreted and detected in the systemic circulation. Different observational clinical studies reported that circulating levels of ANGPTL2 increase significantly in various chronic inflammatory diseases and showed associations between ANGPTL2 levels and diagnosis and/or prognosis of cardiovascular diseases, diabetes, chronic kidney disease, and various types of cancers. |
Product Category |
Recombinant Protein |
Target |
ANGPTL2/Angiopoietin-like 2 |
| Synonyms | ARP2; HARP; angiopoietin-like 2; ANGPTL2; ANGRP2; MGC8889 |
Gene/Protein ID |
Q9UKU9-1 |
Species |
Human |
Exact Sequence |
Thr260-His493 |
| Molecular Weight | The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 65.63 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human ANGPTL2, His Tag with an affinity constant of 65.63 nM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Human ANGPTL2 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human ANGPTL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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