- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Biotinylated Human ANGPTL7, His Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 1.41ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: GIn27-Pro346
Product Description |
Recombinant Biotinylated Human ANGPTL7/CDT6 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains GIn27-Pro346. |
Product Background |
Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
ANGPTL7/CDT6 |
Synonyms |
angiopoietin-like 7; Angiopoietin-like factor; ANGPTL7; AngX; CDT6 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
GIn27-Pro346 |
Molecular Weight |
The protein has a predicted MW of 40.2 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human ANGPTL7, His Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 1.41ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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