Biotinylated Human ANGPTL7/CDT6 Protein (ANG-HM407B)
Biotinylated Human ANGPTL7/CDT6 Protein (ANG-HM407B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human ANGPTL7, His Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 1.41ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: GIn27-Pro346
Catalog #ANG-HM407B
Regular price $578.00
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Product Description

Recombinant Biotinylated Human ANGPTL7/CDT6 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains GIn27-Pro346.

Product Background

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
ANGPTL7/CDT6
Synonyms
angiopoietin-like 7; Angiopoietin-like factor; ANGPTL7; AngX; CDT6
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
GIn27-Pro346
Molecular Weight
The protein has a predicted MW of 40.2 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human ANGPTL7, His Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 1.41ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human ANGPTL7, His Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 1.41ug/ml determined by ELISA. Immobilized Biotinylated Human ANGPTL7, His Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 1.41ug/ml determined by ELISA.
Biotinylated Human ANGPTL7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ANGPTL7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ANGPTL7 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human ANGPTL7 is greater than 95% as determined by SEC-HPLC.
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