Name: Biotinylated Human APRIL/TNFSF13 Trimer Protein (APR-HM410B)
Brand: KactusBio
SKU: APR-HM410B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human APRIL/TNFSF13 Trimer Protein (APR-HM410B)

Express System: HEK293
Product Tag: N-His-Avi-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Lys112-Leu250

Catalog #APR-HM410B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag, Avi tag and Flag Tag at the N-Terminus.It contains Lys112-Leu250.
Product Background	The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Product Category	Recombinant Protein

Target	APRIL/TNFSF13 Trimer
Accession	O75888-1
Species	Human
Biotinylated	yes
Amino Acid Range	Lys112-Leu250
Molecular Weight	The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi-Flag
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA.
	Biotinylated Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	The purity of Biotinylated Human APRIL is greater than 95% as determined by SEC-HPLC.

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