Name: Biotinylated Human APRIL/TNFSF13 Trimer Protein (APR-HM410B)
Brand: KactusBio
SKU: APR-HM410B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human APRIL/TNFSF13 Trimer Protein (APR-HM410B)

Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA.

Biotinylated Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Biotinylated Human APRIL (Trimer), His-Avi-Flag Tag with an affinity constant of 0.67nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human APRIL Trimer is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA.
Amino Acid Range: Lys112-Leu250

Catalog #APR-HM410B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human APRIL/TNFSF13 Trimer Protein (APR-HM410B) Summary

Product Description	Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag, Avi tag and Flag Tag at the N-Terminus.It contains Lys112-Leu250 trimer design.
Product Background	The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human APRIL/TNFSF13 Trimer Protein (APR-HM410B) Specifications/Details

Protein	APRIL/TNFSF13
Synonyms	CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
Accession	O75888-1
Species	Human
Biotinylated	yes
Amino Acid Range	Lys112-Leu250
Molecular Weight	The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA.
Product Tag	N-His-Avi-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA.
	Biotinylated Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Biotinylated Human APRIL (Trimer), His-Avi-Flag Tag with an affinity constant of 0.67nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human APRIL Trimer is greater than 90% as determined by SEC-HPLC.