Product Description |
Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag, Avi tag and Flag Tag at the N-Terminus.It contains Lys112-Leu250. |
Product Background |
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. |
Product Category |
Recombinant Protein |
Target |
APRIL/TNFSF13 Trimer |
Synonyms |
CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Lys112-Leu250 |
Molecular Weight |
The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA. |
|
|
Biotinylated Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
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The purity of Biotinylated Human APRIL is greater than 95% as determined by SEC-HPLC. |
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