Product Description |
Recombinant Biotinylated Human ASGR1 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Gln62-Leu291. |
Product Background |
ASGR1 is a liver-specific surface marker that is exclusively expressed in liver and mediates internalization and lysosomal degradation of blood asialoglycoproteins. |
Product Category |
Recombinant Protein |
Target |
ASGR1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln62-Leu291 |
Molecular Weight |
The protein has a predicted MW of 29.30 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human ASGR1, His Tag captured on CM5 Chip via Anti-his antibody can bind SARS-COV-2 Spike S1, hFc Tag with an affinity constant of 0.132 uM as determined in SPR assay (Biacore T200) (QC Test). |
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Biotinylated Human ASGR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human ASGR1 is greater than 95% as determined by SEC-HPLC. |
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