Biotinylated Human ASGR1 Protein (ASG-HM4R1B)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln62-Leu291

Catalog: ASG-HM4R1B

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Product Description	Recombinant Biotinylated Human ASGR1 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Gln62-Leu291.
Product Background	ASGR1 is a liver-specific surface marker that is exclusively expressed in liver and mediates internalization and lysosomal degradation of blood asialoglycoproteins.
Product Category	Recombinant Protein

Target	ASGR1
Accession	P07306-1
Species	Human
Amino Acid Range	Gln62-Leu291
Molecular Weight	The protein has a predicted MW of 29.30 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human ASGR1, His Tag captured on CM5 Chip via Protein A can bind SARS-COV-2 Spike S1, hFc Tag with an affinity constant of 0.132 uM as determined in SPR assay (Biacore T200).
	The purity of Biotinylated Human ASGR1 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human ASGR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.