Name: Biotinylated Human B7-1/CD80 Protein (B71-HM480B)
Brand: KactusBio
SKU: B71-HM480B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human B7-1/CD80 Protein (B71-HM480B)

Immobilized Biotinylated Human B7-1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.

Biotinylated Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of-Biotinylated Human B7-1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human B7-1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Val35-Asn242

Catalog #B71-HM480B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val35-Asn242.
Product Background	Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	B7-1/CD80
Synonyms	CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
Accession	P33681-1
Species	Human
Biotinylated	yes
Amino Acid Range	Val35-Asn242
Molecular Weight	The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human B7-1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human B7-1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
	Biotinylated Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of-Biotinylated Human B7-1 is greater than 95% as determined by SEC-HPLC.