Biotinylated Human B7-1/CD80 Protein (B71-HM480B)
Catalog: B71-HM480B
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| Product Description | Recombinant Biotinylated Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val35-Asn242. |
Product Background |
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. |
Product Category |
Recombinant Protein |
Target |
B7-1/CD80 |
| Synonyms | CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7 |
Gene/Protein ID |
P33681-1 |
Species |
Human |
Exact Sequence |
Val35-Asn242 |
| Molecular Weight | The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human B7-1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human B7-1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. |
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The purity of-Biotinylated Human B7-1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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