Biotinylated Human B7-2/CD86 Protein (B72-HM486B)
Biotinylated Human B7-2/CD86 Protein (B72-HM486B)
Biotinylated Human B7-2/CD86 Protein (B72-HM486B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human B7-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu26-Pro247
Catalog #B72-HM486B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human B7-2/CD86 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Pro247.
Product Background
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Product Category
Recombinant Protein
Target
B7-2/CD86
Synonyms
CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu26-Pro247
Molecular Weight
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human B7-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human B7-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
Biotinylated Human B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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