Name: Biotinylated Human B7-2/CD86 Protein (B72-HM486B)
Brand: KactusBio
SKU: B72-HM486B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human B7-2/CD86 Protein (B72-HM486B)

Immobilized Biotinylated Human B7-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.

Biotinylated Human B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human B7-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu26-Pro247

Catalog #B72-HM486B

Size

100UG

500UG

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Biotinylated Human B7-2/CD86 Protein (B72-HM486B) Summary

Product Description	Recombinant Biotinylated Human B7-2/CD86 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Pro247.
Product Background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human B7-2/CD86 Protein (B72-HM486B) Specifications/Details

Protein	B7-2/CD86
Synonyms	CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
Accession	P42081-1
Species	Human
Biotinylated	yes
Amino Acid Range	Leu26-Pro247
Molecular Weight	The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human B7-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human B7-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
	Biotinylated Human B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.