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Biotinylated Human B7-H4 Protein, Ultra Low Endotoxin (BH7-HM174B-UL) Summary
Product Description |
Recombinant Biotinylated Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258. |
Product Background |
B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins | ADC targets |
Biotinylated Human B7-H4 Protein, Ultra Low Endotoxin (BH7-HM174B-UL) Specifications/Details
Protein |
B7-H4 |
Synonyms |
B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe29-Ala258 |
Molecular Weight |
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human B7-H4, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human B7-H4, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). |
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Biotinylated Human B7-H4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human B7-H4 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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