Name: Biotinylated Human B7-H5/Gi24/VISTA Protein (BH7-HM475B)
Brand: KactusBio
SKU: BH7-HM475B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human B7-H5/Gi24/VISTA Protein (BH7-HM475B)

Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

Biotinylated Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human B7-H5 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Amino Acid Range: Phe33-Ala194

Catalog #BH7-HM475B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human B7-H5/Gi24/VISTA Protein (BH7-HM475B) Summary

Product Description	Recombinant Biotinylated Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Phe33-Ala194.
Product Background	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human B7-H5/Gi24/VISTA Protein (BH7-HM475B) Specifications/Details

Protein	B7-H5/Gi24/VISTA
Synonyms	B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1
Accession	Q9H7M9
Species	Human
Biotinylated	yes
Amino Acid Range	Phe33-Ala194
Molecular Weight	The protein has a predicted MW of 21.04 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
	Biotinylated Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human B7-H5 is greater than 95% as determined by SEC-HPLC.