Biotinylated Human B7-H5/Gi24/VISTA Protein (BH7-HM475B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human B7-H5, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe33-Ala194

Catalog: BH7-HM475B

$525.00
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Product Description Recombinant Biotinylated Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Phe33-Ala194.

Product Background

B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.

Product Category

Recombinant Protein

Target

B7-H5/Gi24/VISTA

Gene/Protein ID

Q9H7M9

Species

Human

Exact Sequence

Phe33-Ala194
Molecular Weight The protein has a predicted MW of 21.04 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human B7-H5, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human B7-H5, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
The purity of Biotinylated Human B7-H5 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human B7-H5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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