- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Phe33-Ala194
Product Description |
Recombinant Biotinylated Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Phe33-Ala194. |
Product Background |
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
B7-H5/Gi24/VISTA |
Synonyms |
B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe33-Ala194 |
Molecular Weight |
The protein has a predicted MW of 21.04 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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