Biotinylated Human B7-H5/Gi24/VISTA Protein (BH7-HM475B)
Biotinylated Human B7-H5/Gi24/VISTA Protein (BH7-HM475B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe33-Ala194
Catalog #BH7-HM475B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Phe33-Ala194.

Product Background

B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

B7-H5/Gi24/VISTA

Synonyms

B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Phe33-Ala194

Molecular Weight

The protein has a predicted MW of 21.04 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. Immobilized Biotinylated Human B7-H5, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Biotinylated Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human B7-H5 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human B7-H5 is greater than 95% as determined by SEC-HPLC.

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