
- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: The affinity constant of 0.227 uM as determined in SPR assay (Biacore T200).
- Amino Acid Range: Asp25-Ser262
Product Description |
Recombinant Biotinylated Human B7-H6/NCR3LG1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp25-Ser262. |
Product Background |
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
B7-H6/NCR3LG1 |
Synonyms |
B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp25-Ser262 |
Molecular Weight |
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.227 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.