Name: Biotinylated Human B7-H6/NCR3LG1 Protein (BH7-HM476B)
Brand: KactusBio
SKU: BH7-HM476B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human B7-H6/NCR3LG1 Protein (BH7-HM476B)

Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human B7-H6, His-Avi Tag with an affinity constant of 0.227 uM as determined in SPR assay (Biacore T200).

Biotinylated Human B7-H6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human B7-H6 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.227 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Asp25-Ser262

Catalog #BH7-HM476B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human B7-H6/NCR3LG1 Protein (BH7-HM476B) Summary

Product Description	Recombinant Biotinylated Human B7-H6/NCR3LG1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp25-Ser262.
Product Background	B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human B7-H6/NCR3LG1 Protein (BH7-HM476B) Specifications/Details

Protein	B7-H6/NCR3LG1
Synonyms	B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6
Accession	Q68D85
Species	Human
Biotinylated	yes
Amino Acid Range	Asp25-Ser262
Molecular Weight	The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.227 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human B7-H6, His-Avi Tag with an affinity constant of 0.227 uM as determined in SPR assay (Biacore T200).
	Biotinylated Human B7-H6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human B7-H6 is greater than 95% as determined by SEC-HPLC.