Biotinylated Human B7-H7/HHLA2 Protein (BH7-HM477B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ile23-Asn344
Catalog #BH7-HM477B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile23-Asn344.

Product Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

B7-H7/HHLA2

Synonyms

B7H7; B7-H7; HHLA2; B7 Homolog 7

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Ile23-Asn344

Molecular Weight

The protein has a predicted MW of 39.88 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Biotinylated Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human B7-H7 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human B7-H7 is greater than 95% as determined by SEC-HPLC.

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