Biotinylated Human B7-H7/HHLA2 Protein (BH7-HM477B)
|Recombinant Biotinylated Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile23-Asn344.
|B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
|The protein has a predicted MW of 39.88 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
|Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped with dry ice.
|Supplied as 0.22 um filtered solution in PBS (pH 7.4).
|Stability And Storage
|Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.