Biotinylated Human B7-H7/HHLA2 Protein (BH7-HM477B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ile23-Asn344
Catalog #BH7-HM477B
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Product Description
 Recombinant Biotinylated Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile23-Asn344.
Product Background
 B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Product Category
 Recombinant Protein
Target
 B7-H7/HHLA2
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ile23-Asn344
Molecular Weight
 The protein has a predicted MW of 39.88 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Biotinylated Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human B7-H7 is greater than 95% as determined by SEC-HPLC.

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