Biotinylated Human BAFF/TNFSF13B/CD257 Protein (BAF-HM4AFB)
|Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala134-Leu285.
|B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
|The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-27 kDa based on Tris-Bis PAGE result.
|Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.