Biotinylated Human BAFF/TNFSF13B/CD257 Protein (BAF-HM4AFB)
Catalog: BAF-HM4AFB
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Product Description | Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala134-Leu285. |
Product Background |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. |
Product Category |
Recombinant Protein |
Target |
BAFF/TNFSF13B/CD257 |
Accession | |
Species |
Human |
Amino Acid Range |
Ala134-Leu285 |
Molecular Weight | The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-27 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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