Biotinylated Human BAFF/TNFSF13B/CD257 Protein (BAF-HM4AFB)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala134-Leu285
Catalog #BAF-HM4AFB
Regular price $525.00
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Product Description

Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala134-Leu285.

Product Background

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Product Category

Recombinant Protein / Antibody discovery

Protein

BAFF/TNFSF13B/CD257

Synonyms

BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Ala134-Leu285

Molecular Weight

The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-27 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).
Immobilized Human BCMA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 6.6ng/ml determined by ELISA. Immobilized Human BCMA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 6.6ng/ml determined by ELISA.
Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC.

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