Name: Biotinylated Human BAFF/TNFSF13B/CD257 Protein (active trimer) (BAF-HM4AFB)
Brand: KactusBio
SKU: BAF-HM4AFB-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human BAFF/TNFSF13B/CD257 Protein (active trimer) (BAF-HM4AFB)

Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).

Immobilized Human BCMA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1ng/ml determined by ELISA.

Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human BAFF is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS.

Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala134-Leu285

Catalog #BAF-HM4AFB

Size

100UG

500UG

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Biotinylated Human BAFF/TNFSF13B/CD257 Protein (active trimer) (BAF-HM4AFB) Summary

Product Description	Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala134-Leu285.
Product Background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human BAFF/TNFSF13B/CD257 Protein (active trimer) (BAF-HM4AFB) Specifications/Details

Protein	BAFF/TNFSF13B/CD257
Synonyms	BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession	Q9Y275-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ala134-Leu285
Molecular Weight	The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
	Immobilized Human BCMA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1ng/ml determined by ELISA.
	Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human BAFF is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS.
	Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC.