• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala134-Leu285
Catalog #BAF-HM4AFB
Price
Regular price $577.50
Regular price Sale price $577.50
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human BAFF/TNFSF13B/CD257 Protein (active trimer) (BAF-HM4AFB) Summary

Product Description

 Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala134-Leu285.

Product Background

 B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human BAFF/TNFSF13B/CD257 Protein (active trimer) (BAF-HM4AFB) Specifications/Details

Protein
 BAFF/TNFSF13B/CD257
Synonyms
 BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1;TALL1delta BAFF; TALL1Delta4BAFF
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala134-Leu285
Molecular Weight
 The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
Product Tag
 N-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
Immobilized Human BCMA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1ng/ml determined by ELISA. Immobilized Human BCMA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1ng/ml determined by ELISA.
Immobilized Biotinylated Human BAFF, His Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-BAFF Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. Immobilized Biotinylated Human BAFF, His Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-BAFF Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human BAFF is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS. The purity of Biotinylated Human BAFF is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS.
Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay . Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay .
The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...