Name: Biotinylated Human BAFFR/TNFRSF13C Protein (BAF-HM40RB)
Brand: KactusBio
SKU: BAF-HM40RB-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human BAFFR/TNFRSF13C Protein (BAF-HM40RB)

Immobilized Human BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 0.42ug/ml determined by ELISA (QC Test).

Immobilized Anti-BAFFR Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 5.5ng/ml determined by ELISA.

Biotinylated Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Loaded Biotinylated Human BAFFR, His-Avi Tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator® Prime).

Loaded Biotinylated Human BAFFR, His-Avi Tag on Anti-His-Biosensor can bind Anti-BAFFR Ab., hFc Tag with an affinity constant of 18.5 pM as determined in BLI assay (Gator® Prime).

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 0.42ug/ml determined by ELISA (QC Test).
Amino Acid Range: Ser7-Ala71

Catalog #BAF-HM40RB

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human BAFFR/TNFRSF13C Protein (BAF-HM40RB) Summary

Product Description	Recombinant Biotinylated Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser7-Ala71.
Product Background	BAFFR is an atypical representative of the TNF-receptor super-family. BAFFR [BAFFR = B cell activating f actor of the TNF-f amily receptor; a.k.a. BR3, Bcmd] expression starts when the immature B cells develop to transitional B cells, which then receive BAFFR-dependent pro-survival signals to rescue them from premature cell death. At protein level, the BAFFR is expressed on the surface of all human peripheral B cell subsets except for plasma cells and for centroblasts located in the dark zone of germinal centers. Its expression is upregulated after the expression of functional B cell antigen receptors (BCR) in response to tonic BCR signaling, which enhances BAFFR expression by immature and transitional B cells
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human BAFFR/TNFRSF13C Protein (BAF-HM40RB) Specifications/Details

Protein	BAFFR/TNFRSF13C
Synonyms	CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
Accession	Q96RJ3-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ser7-Ala71
Molecular Weight	The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 0.42ug/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 0.42ug/ml determined by ELISA (QC Test).
	Immobilized Anti-BAFFR Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 5.5ng/ml determined by ELISA.
	Biotinylated Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	Loaded Biotinylated Human BAFFR, His-Avi Tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator® Prime).
	Loaded Biotinylated Human BAFFR, His-Avi Tag on Anti-His-Biosensor can bind Anti-BAFFR Ab., hFc Tag with an affinity constant of 18.5 pM as determined in BLI assay (Gator® Prime).