Product Description |
Recombinant Biotinylated Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser7-Ala71. |
Product Background |
BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. |
Product Category |
Recombinant Protein |
Target |
BAFFR/TNFRSF13C |
Synonyms |
CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser7-Ala71 |
Molecular Weight |
The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-BAFFR Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 5.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Anti-BAFFR Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 5.5ng/ml determined by ELISA. |
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Immobilized Human BAFF (Trimer) , His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 53.5ng/ml determined by ELISA (QC Test). |
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Biotinylated Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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