Product Description |
Recombinant Biotinylated Human BDCA-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Phe46-Ile213. |
Product Background |
BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling. |
Product Category |
Recombinant Protein |
Target |
BDCA-2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe46-Ile213 |
Molecular Weight |
The protein has a predicted MW of 22.7 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human BDCA-2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in 20mM Tris, 300mM NaCl, 300mM L-arginine (pH 8.5). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human BDCA-2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. |
|
|
Biotinylated Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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