Biotinylated Human BDCA-2 Protein (BCA-HM402B)
Biotinylated Human BDCA-2 Protein (BCA-HM402B)
Biotinylated Human BDCA-2 Protein (BCA-HM402B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human BDCA-2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe46-Ile213
Catalog #BCA-HM402B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human BDCA-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Phe46-Ile213.
Product Background
BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
Product Category
Recombinant Protein
Target
BDCA-2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Phe46-Ile213
Molecular Weight
The protein has a predicted MW of 22.7 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human BDCA-2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in 20mM Tris, 300mM NaCl, 300mM L-arginine (pH 8.5).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human BDCA-2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Biotinylated Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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