Name: Biotinylated Human BDCA-2 Protein (BCA-HM402B)
Brand: KactusBio
SKU: BCA-HM402B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human BDCA-2 Protein (BCA-HM402B)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human BDCA-2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Phe46-Ile213

Catalog #BCA-HM402B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human BDCA-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Phe46-Ile213.
Product Background	BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
Product Category	Recombinant Protein

Target	BDCA-2
Synonyms	BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303; CD303; DLEC;
Accession	Q8WTT0-1
Species	Human
Biotinylated	yes
Amino Acid Range	Phe46-Ile213
Molecular Weight	The protein has a predicted MW of 22.7 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human BDCA-2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM Tris, 300mM NaCl, 300mM L-arginine (pH 8.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human BDCA-2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
	Biotinylated Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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