Biotinylated Human BTN3A1/CD277 Protein (BTN-HM4A3B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BTN3A1, His Tag with the EC50 of 15.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln30-Gly254

Catalog: BTN-HM4A3B

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Product Description Recombinant Biotinylated Human BTN3A1/CD277 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln30-Gly254.
Product Category Recombinant Protein
Target BTN3A1/CD277
Synonyms CD277; BTN3A1; BTF5; BT3.1; BTN3.1
Gene/Protein ID O00481-1
Species Human
Exact Sequence Gln30-Gly254
Molecular Weight The protein has a predicted MW of 27.1 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BTN3A1, His Tag with the EC50 of 15.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BTN3A1, His Tag with the EC50 of 15.4ng/ml determined by ELISA.
The purity of Biotinylated Human BTN3A1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human BTN3A1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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