Name: Biotinylated Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401B)
Brand: KactusBio
SKU: CA9-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401B)

Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.

Biotinylated Human CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CA9 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.Measured by its esterase activity. The specific activity is >50 pmol/min/µg.
Amino Acid Range: Gln38-Asp414

Catalog #CA9-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401B) Summary

Product Description	Recombinant Biotinylated Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Gln38-Asp414.
Product Background	CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401B) Specifications/Details

Protein	CA9/Carbonic Anhydrase IX
Synonyms	CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC
Accession	Q16790
Species	Human
Biotinylated	yes
Amino Acid Range	Gln38-Asp414
Molecular Weight	The protein has a predicted MW of 43.7 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.Measured by its esterase activity. The specific activity is >50 pmol/min/µg.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.
	Biotinylated Human CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CA9 is greater than 95% as determined by SEC-HPLC.