Biotinylated Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.Measured by its esterase activity. The specific activity is >50 pmol/min/µg.
Amino Acid Range: Gln38-Asp414

Catalog #CA9-HM401B

Price

$577⁵⁰

Size

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Biotinylated Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401B) Summary

Product Description	Recombinant Biotinylated Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Gln38-Asp414.
Product Background	CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.

Protein	CA9/Carbonic Anhydrase IX
Synonyms	CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC;EC4.2.1.1
Accession	Q16790
Species	Human
Biotinylated	yes
Amino Acid Range	Gln38-Asp414
Molecular Weight	The protein has a predicted MW of 43.7 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.Measured by its esterase activity. The specific activity is >50 pmol/min/µg.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.
	Biotinylated Human CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CA9 is greater than 95% as determined by SEC-HPLC.

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