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Biotinylated Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401B) Summary
Product Description |
Recombinant Biotinylated Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Gln38-Asp414. |
Product Background |
CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401B) Specifications/Details
Protein |
CA9/Carbonic Anhydrase IX |
Synonyms |
CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln38-Asp414 |
Molecular Weight |
The protein has a predicted MW of 43.7 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.Measured by its esterase activity. The specific activity is >50 pmol/min/µg. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human CA9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. |
|
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Biotinylated Human CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human CA9 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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