Name: Biotinylated Human CCL5 Protein (CCL-HE405B)
Brand: KactusBio
SKU: CCL-HE405B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve showing the dose-dependent interaction between biotinylated Human CCL5, His Tag, and Anti-CCL5 Antibody, hFc Tag. The plot presents mean absorbance (OD450) versus the log concentration of the biotinylated CCL5 protein (µg/ml), displaying a sigmoidal response.

Biotinylated Human CCL5 Protein (CCL-HE405B)

Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).

Biotinylated Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Amino Acid Range: Ser24-Ser91

Catalog #CCL-HE405B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CCL5 Protein (CCL-HE405B) Summary

Product Description	Recombinant Biotinylated Human CCL5 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ser24-Ser91.
Product Background	The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CCL5 Protein (CCL-HE405B) Specifications/Details

Protein	CCL5
Synonyms	MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5
Accession	P13501
Species	Human
Biotinylated	yes
Amino Acid Range	Ser24-Ser91
Molecular Weight	The protein has a predicted MW of 10.89 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Product Tag	N-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.