Biotinylated Human CCL5 Protein (CCL-HE405B)

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Biotinylated: yes
Activity: Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser24-Ser91

Catalog: CCL-HE405B

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Product Description	Recombinant Biotinylated Human CCL5 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ser24-Ser91.
Product Background	The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
Product Category	Recombinant Protein

Target	CCL5
Synonyms	MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5
Gene/Protein ID	P13501
Species	Human
Exact Sequence	Ser24-Ser91
Molecular Weight	The protein has a predicted MW of 10.89 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 0.3%SKL (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
	Biotinylated Human CCL5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.