• Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ser24-Ser91
Catalog #CCL-HE405B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human CCL5 Protein (CCL-HE405B) Summary

Product Description

 Recombinant Biotinylated Human CCL5 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ser24-Ser91.

Product Background

 The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.

Biotinylated Human CCL5 Protein (CCL-HE405B) Specifications/Details

Protein
 CCL5
Synonyms
 MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ser24-Ser91
Molecular Weight
 The protein has a predicted MW of 10.89 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Biotinylated Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...