• Express System: HEK293
  • Purity: > 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CCR2b VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CCR2 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 2.19 nM as determined in SPR assay (Biacore T200).
  • Negative Control: Biotinylated VLP Control (GPR-HM05CB)
  • Amino Acid Range: Met1-Leu360
Catalog #CCR-HM02BB
Price
Regular price $1,76500
Regular price Sale price $1,76500
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human CCR2b VLP (CCR-HM02BB) Summary

Product Description

 Recombinant Biotinylated Human CCR2b VLP is expressed from HEK293.It contains Met1-Leu360 (It may have cross reaction with anti-His antibody).

Product Background

 The chemokine (C-C motif) receptor 2B (CCR2B) is one of the two isoforms of the receptor for monocyte chemoattractant protein-1 (CCL2), the major chemoattractant for monocytes, involved in an array of chronic inflammatory diseases. The actin-binding protein filamin A (FLNa) as a protein that associates with the carboxyl-terminal tail of CCR2B. FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures.

Biotinylated Human CCR2b VLP (CCR-HM02BB) Specifications/Details

Protein
 CCR2b
Synonyms
 C-C CKR-2; CCR-2; CCR2; MCP-1-R; CMKBR2; CCR2;C-C CKR2; MCP1R
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met1-Leu360
Molecular Weight
 The target protein has a predicted MW of 42.9kDa.
Activity
 Immobilized Biotinylated Human CCR2b VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CCR2 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 2.19 nM as determined in SPR assay (Biacore T200).
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS, 300mM L-arginine (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated CCR2B VLP captured on CM5 Chip via streptavidin can bind Anti-CCR2 Antibody, hFc with an affinity constant of 2.19 nM as determined in SPR assay (Biacore T200). Biotinylated CCR2B VLP captured on CM5 Chip via streptavidin can bind Anti-CCR2 Antibody, hFc with an affinity constant of 2.19 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CCR2b VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CCR2 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CCR2b VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CCR2 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).
The purity of Biotinylated Human CCR2b VLP is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CCR2b VLP is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...